Composite
Part:BBa_K4343067:Design
Designed by: Xiaolu Sun Group: iGEM22_NNU-China (2022-10-09)
pUC-HUH-EgDes8
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 4994
Illegal XhoI site found at 1920
Illegal XhoI site found at 1953
Illegal XhoI site found at 5315 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2108
Illegal AgeI site found at 97
Illegal AgeI site found at 458
Illegal AgeI site found at 3161
Illegal AgeI site found at 3522
Illegal AgeI site found at 4664
Illegal AgeI site found at 5955 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI site found at 638
Illegal BsaI site found at 3702
Illegal BsaI site found at 4239
Illegal BsaI site found at 4667
Illegal BsaI site found at 5958
Illegal BsaI.rc site found at 4661
Illegal BsaI.rc site found at 5952
Illegal BsaI.rc site found at 6222
Illegal SapI.rc site found at 363
Illegal SapI.rc site found at 3427
Illegal SapI.rc site found at 4939
Design Notes
NONE
Source
from Euglena gracilis